Service summary
- RNA extractions with QIAsymphony Paxgene Blood RNA Kit on QIAsymphony for unextracted samples
- QC check with Invitrogen Qubit, Agilent Bioanalyzer and Tapestation
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq 6000, ≥25 million reads (R1 + R2 = 50 M Reads) output per sample
- Turnover time approx. 4 to 6 weeks. Turnover times are not guaranteed, may change according to your project.
What we do with your samples
- Once your samples arrive at our laboratories, we start by performing a reception control step in which we make sure the samples meet our requirements.
- If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, we will only make one attempt at library preparation.
- If the samples pass reception control, we will inform you and the samples will be queued for library preparation.
Library preparation
- Illumina Stranded total RNA Prep (Ribo-Zero Plus) Kit is used. The total-RNA samples are depleted from abundant transcripts, such as ribosomal RNA, using Ribo-Zero Plus depletion reagents. The samples are then chemically fragmented and turned into cDNA using reverse transcriptase, while subsequent ligation and amplification steps add the Illumina index-adapters for clustering and sequencing.
- Total RNA-sequencing with ribosomal depletion offers a comprehensive whole transcriptome analysis by capturing diverse RNA molecules (not small RNA) such as mRNAs, non-coding RNAs that are not polyadenylated and unspliced transcripts.
- The concentration of the libraries are analyzed using qPCR and the fragment size distribution of the library molecules are using Bioanalyzer.
Sequencing & Analysis
- Raw sequencing data is first generated on Illumina NovaSeq 6000 instrument.
- Illumina DRAGEN Bio-IT Platform leverages for accurate, rapid secondary analysis
Output
- As a standard set of final output, we provide FASTQ files in tandem with BAM files
- Final output files are shared via WeTransfer link.
Image by kjpargeter on Freepik.
본 서비스는 고객님의 시퀀싱 분석을 진행하는 맞춤형 서비스로, 시퀀싱 작업의 특성상 반품, 교환 및 환불이 제한됩니다.
- 반품 및 환불 불가: 시퀀싱 분석 서비스는 첫 QC(Quality Control) 완료 후 라이브러리 준비 전, 고객님의 확인을 거쳐 진행되므로, 해당 시점 이후에는 반품 및 환불이 불가합니다.
- 교환 정책: 시퀀싱 진행 중 예기치 않은 품질 문제 발생 시, 내부 검토를 거쳐 동일한 분석 조건에서 재진행 가능 여부를 검토합니다.
서비스 이용에 관한 문의는 고객 지원팀으로 연락 주시기 바랍니다.
Service summary
- RNA extractions with QIAsymphony Paxgene Blood RNA Kit on QIAsymphony for unextracted samples
- QC check with Invitrogen Qubit, Agilent Bioanalyzer and Tapestation
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq 6000, ≥25 million reads (R1 + R2 = 50 M Reads) output per sample
- Turnover time approx. 4 to 6 weeks. Turnover times are not guaranteed, may change according to your project.
What we do with your samples
- Once your samples arrive at our laboratories, we start by performing a reception control step in which we make sure the samples meet our requirements.
- If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, we will only make one attempt at library preparation.
- If the samples pass reception control, we will inform you and the samples will be queued for library preparation.
Library preparation
- Illumina Stranded total RNA Prep (Ribo-Zero Plus) Kit is used. The total-RNA samples are depleted from abundant transcripts, such as ribosomal RNA, using Ribo-Zero Plus depletion reagents. The samples are then chemically fragmented and turned into cDNA using reverse transcriptase, while subsequent ligation and amplification steps add the Illumina index-adapters for clustering and sequencing.
- Total RNA-sequencing with ribosomal depletion offers a comprehensive whole transcriptome analysis by capturing diverse RNA molecules (not small RNA) such as mRNAs, non-coding RNAs that are not polyadenylated and unspliced transcripts.
- The concentration of the libraries are analyzed using qPCR and the fragment size distribution of the library molecules are using Bioanalyzer.
Sequencing & Analysis
- Raw sequencing data is first generated on Illumina NovaSeq 6000 instrument.
- Illumina DRAGEN Bio-IT Platform leverages for accurate, rapid secondary analysis
Output
- As a standard set of final output, we provide FASTQ files in tandem with BAM files
- Final output files are shared via WeTransfer link.
Image by kjpargeter on Freepik.
본 서비스는 고객님의 시퀀싱 분석을 진행하는 맞춤형 서비스로, 시퀀싱 작업의 특성상 반품, 교환 및 환불이 제한됩니다.
- 반품 및 환불 불가: 시퀀싱 분석 서비스는 첫 QC(Quality Control) 완료 후 라이브러리 준비 전, 고객님의 확인을 거쳐 진행되므로, 해당 시점 이후에는 반품 및 환불이 불가합니다.
- 교환 정책: 시퀀싱 진행 중 예기치 않은 품질 문제 발생 시, 내부 검토를 거쳐 동일한 분석 조건에서 재진행 가능 여부를 검토합니다.
서비스 이용에 관한 문의는 고객 지원팀으로 연락 주시기 바랍니다.
