Service summary
- DNA extractions with Qiagen kits on QIAsymphony for unextracted samples
- QC check with Victor Nivo Multimode Microplate Reader and Agilent Bioanalyzer
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq 6000 with 100x genome coverage (8GB output per sample)
- Turnover time approx. 4 to 6 weeks. Turnover times are not guaranteed, may change according to your project.
What we do with your samples
- Once your samples arrive at our laboratories, we start by performing a reception control step in which we make sure the samples meet our requirements.
- If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, we will only make one attempt at library preparation.
- If the samples pass reception control, we will inform you and the samples will be queued for library preparation.
Library preparation
- Illumina DNA Prep with Enrichment kit is used for the whole exome sequencing. The protocol for library preparation involves DNA fragmentation and ligation of Illumina adapters with sample-specific barcodes. After sample and library preparation, a hybridization-based target enrichment step is performed using probes that contain specific sequences that are designed to bind complementary to the exome DNA sequences.
- We evaluate the concentration of the libraries using qPCR and look at the fragment size distribution of the library molecules using Bioanalyzer.
Sequencing & Analysis
- Raw sequencing data is first generated on Illumina NovaSeq 6000 instrument.
- Illumina DRAGEN Bio-IT Platform leverages for accurate, rapid secondary analysis
Output
- As a standard set of final output, we provide FASTQ files in tandem with BAM files
- Final output files are shared via WeTransfer link.
Image by kjpargeter from Freepik
본 서비스는 고객님의 시퀀싱 분석을 진행하는 맞춤형 서비스로, 시퀀싱 작업의 특성상 반품, 교환 및 환불이 제한됩니다.
- 반품 및 환불 불가: 시퀀싱 분석 서비스는 첫 QC(Quality Control) 완료 후 라이브러리 준비 전, 고객님의 확인을 거쳐 진행되므로, 해당 시점 이후에는 반품 및 환불이 불가합니다.
- 교환 정책: 시퀀싱 진행 중 예기치 않은 품질 문제 발생 시, 내부 검토를 거쳐 동일한 분석 조건에서 재진행 가능 여부를 검토합니다.
서비스 이용에 관한 문의는 고객 지원팀으로 연락 주시기 바랍니다.
Service summary
- DNA extractions with Qiagen kits on QIAsymphony for unextracted samples
- QC check with Victor Nivo Multimode Microplate Reader and Agilent Bioanalyzer
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq 6000 with 100x genome coverage (8GB output per sample)
- Turnover time approx. 4 to 6 weeks. Turnover times are not guaranteed, may change according to your project.
What we do with your samples
- Once your samples arrive at our laboratories, we start by performing a reception control step in which we make sure the samples meet our requirements.
- If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, we will only make one attempt at library preparation.
- If the samples pass reception control, we will inform you and the samples will be queued for library preparation.
Library preparation
- Illumina DNA Prep with Enrichment kit is used for the whole exome sequencing. The protocol for library preparation involves DNA fragmentation and ligation of Illumina adapters with sample-specific barcodes. After sample and library preparation, a hybridization-based target enrichment step is performed using probes that contain specific sequences that are designed to bind complementary to the exome DNA sequences.
- We evaluate the concentration of the libraries using qPCR and look at the fragment size distribution of the library molecules using Bioanalyzer.
Sequencing & Analysis
- Raw sequencing data is first generated on Illumina NovaSeq 6000 instrument.
- Illumina DRAGEN Bio-IT Platform leverages for accurate, rapid secondary analysis
Output
- As a standard set of final output, we provide FASTQ files in tandem with BAM files
- Final output files are shared via WeTransfer link.
Image by kjpargeter from Freepik
본 서비스는 고객님의 시퀀싱 분석을 진행하는 맞춤형 서비스로, 시퀀싱 작업의 특성상 반품, 교환 및 환불이 제한됩니다.
- 반품 및 환불 불가: 시퀀싱 분석 서비스는 첫 QC(Quality Control) 완료 후 라이브러리 준비 전, 고객님의 확인을 거쳐 진행되므로, 해당 시점 이후에는 반품 및 환불이 불가합니다.
- 교환 정책: 시퀀싱 진행 중 예기치 않은 품질 문제 발생 시, 내부 검토를 거쳐 동일한 분석 조건에서 재진행 가능 여부를 검토합니다.
서비스 이용에 관한 문의는 고객 지원팀으로 연락 주시기 바랍니다.
