Service summary
- DNA extractions with Qiagen kits on QIAsymphony for unextracted samples
- QC check with Victor Nivo Multimode Microplate Reader
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq X Plus or NovaSeq 6000, 30x genome coverage
- Turnover time approx. 2 to 3 weeks. Turnover times are not guaranteed, may change according to your project.
What we do with your samples
- Once your samples arrive at our laboratories, we start by performing a reception control step in which we make sure the samples meet our requirements.
- If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, we will only make one attempt at library preparation.
- If the samples pass reception control, we will inform you and the samples will be queued for library preparation.
Library preparation
- Illumina’s new tagmentation-based DNA PCR-free library preparation is the method of choice for generating the highest quality DNA sequencing libraries. PCR-free libraries have better coverage of GC-rich regions compared to PCR-based methods and the reads are more evenly distributed over the genome.
- Library QC and sequencing: We evaluate the concentration of the library and inform you of the QC status of each sample. Once the libraries have passed this QC step, they are normalized, pooled, and queued for sequencing.
Sequencing & Analysis
- Raw sequencing data is first generated on either Illumina NovaSeq X Plus or NovaSeq 6000 instruments.
- Illumina DRAGEN Bio-IT Platform leverages for accurate, rapid secondary analysis
Output
- As a final output, we provide FASTQ files in tandem with BAM files as a standard set of output. If requested specifically by our clients, we can also provide VCF files.
- Final output files are shared via WeTransfer link.
본 서비스는 고객님의 시퀀싱 분석을 진행하는 맞춤형 서비스로, 시퀀싱 작업의 특성상 반품, 교환 및 환불이 제한됩니다.
- 반품 및 환불 불가: 시퀀싱 분석 서비스는 첫 QC(Quality Control) 완료 후 라이브러리 준비 전, 고객님의 확인을 거쳐 진행되므로, 해당 시점 이후에는 반품 및 환불이 불가합니다.
- 교환 정책: 시퀀싱 진행 중 예기치 않은 품질 문제 발생 시, 내부 검토를 거쳐 동일한 분석 조건에서 재진행 가능 여부를 검토합니다.
서비스 이용에 관한 문의는 고객 지원팀으로 연락 주시기 바랍니다.
Service summary
- DNA extractions with Qiagen kits on QIAsymphony for unextracted samples
- QC check with Victor Nivo Multimode Microplate Reader
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq X Plus or NovaSeq 6000, 30x genome coverage
- Turnover time approx. 2 to 3 weeks. Turnover times are not guaranteed, may change according to your project.
What we do with your samples
- Once your samples arrive at our laboratories, we start by performing a reception control step in which we make sure the samples meet our requirements.
- If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, we will only make one attempt at library preparation.
- If the samples pass reception control, we will inform you and the samples will be queued for library preparation.
Library preparation
- Illumina’s new tagmentation-based DNA PCR-free library preparation is the method of choice for generating the highest quality DNA sequencing libraries. PCR-free libraries have better coverage of GC-rich regions compared to PCR-based methods and the reads are more evenly distributed over the genome.
- Library QC and sequencing: We evaluate the concentration of the library and inform you of the QC status of each sample. Once the libraries have passed this QC step, they are normalized, pooled, and queued for sequencing.
Sequencing & Analysis
- Raw sequencing data is first generated on either Illumina NovaSeq X Plus or NovaSeq 6000 instruments.
- Illumina DRAGEN Bio-IT Platform leverages for accurate, rapid secondary analysis
Output
- As a final output, we provide FASTQ files in tandem with BAM files as a standard set of output. If requested specifically by our clients, we can also provide VCF files.
- Final output files are shared via WeTransfer link.
본 서비스는 고객님의 시퀀싱 분석을 진행하는 맞춤형 서비스로, 시퀀싱 작업의 특성상 반품, 교환 및 환불이 제한됩니다.
- 반품 및 환불 불가: 시퀀싱 분석 서비스는 첫 QC(Quality Control) 완료 후 라이브러리 준비 전, 고객님의 확인을 거쳐 진행되므로, 해당 시점 이후에는 반품 및 환불이 불가합니다.
- 교환 정책: 시퀀싱 진행 중 예기치 않은 품질 문제 발생 시, 내부 검토를 거쳐 동일한 분석 조건에서 재진행 가능 여부를 검토합니다.
서비스 이용에 관한 문의는 고객 지원팀으로 연락 주시기 바랍니다.
