Service summary
- DNA extractions with Qiagen kits on QIAsymphony for unextracted samples
- QC check with Victor Nivo Multimode Microplate Reader
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq X Plus or NovaSeq 6000, 30x genome coverage
- Turnover time approx. 2 to 3 weeks. Turnover times are not guaranteed, may change according to your project.
What we do with your samples
- Once your samples arrive at our laboratories, we start by performing a reception control step in which we make sure the samples meet our requirements.
- If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, we will only make one attempt at library preparation.
- If the samples pass reception control, we will inform you and the samples will be queued for library preparation.
Library preparation
- Illumina’s new tagmentation-based DNA PCR-free library preparation is the method of choice for generating the highest quality DNA sequencing libraries. PCR-free libraries have better coverage of GC-rich regions compared to PCR-based methods and the reads are more evenly distributed over the genome.
- Library QC and sequencing: We evaluate the concentration of the library and inform you of the QC status of each sample. Once the libraries have passed this QC step, they are normalized, pooled, and queued for sequencing.
Sequencing & Analysis
- Raw sequencing data is first generated on either Illumina NovaSeq X Plus or NovaSeq 6000 instruments.
- Illumina DRAGEN Bio-IT Platform leverages for accurate, rapid secondary analysis
Output
- As a final output, we provide FASTQ files in tandem with BAM files as a standard set of output. If requested specifically by our clients, we can also provide VCF files.
- Final output files are shared via WeTransfer link.
๋ณธ ์๋น์ค๋ ๊ณ ๊ฐ๋์ ์ํ์ฑ ๋ถ์์ ์งํํ๋ ๋ง์ถคํ ์๋น์ค๋ก, ์ํ์ฑ ์์ ์ ํน์ฑ์ ๋ฐํ, ๊ตํ ๋ฐ ํ๋ถ์ด ์ ํ๋ฉ๋๋ค.
- ๋ฐํ ๋ฐ ํ๋ถ ๋ถ๊ฐ: ์ํ์ฑ ๋ถ์ ์๋น์ค๋ ์ฒซ QC(Quality Control) ์๋ฃ ํ ๋ผ์ด๋ธ๋ฌ๋ฆฌ ์ค๋น ์ , ๊ณ ๊ฐ๋์ ํ์ธ์ ๊ฑฐ์ณ ์งํ๋๋ฏ๋ก, ํด๋น ์์ ์ดํ์๋ ๋ฐํ ๋ฐ ํ๋ถ์ด ๋ถ๊ฐํฉ๋๋ค.
- ๊ตํ ์ ์ฑ : ์ํ์ฑ ์งํ ์ค ์๊ธฐ์น ์์ ํ์ง ๋ฌธ์ ๋ฐ์ ์, ๋ด๋ถ ๊ฒํ ๋ฅผ ๊ฑฐ์ณ ๋์ผํ ๋ถ์ ์กฐ๊ฑด์์ ์ฌ์งํ ๊ฐ๋ฅ ์ฌ๋ถ๋ฅผ ๊ฒํ ํฉ๋๋ค.
์๋น์ค ์ด์ฉ์ ๊ดํ ๋ฌธ์๋ ๊ณ ๊ฐ ์ง์ํ์ผ๋ก ์ฐ๋ฝ ์ฃผ์๊ธฐ ๋ฐ๋๋๋ค.
Service summary
- DNA extractions with Qiagen kits on QIAsymphony for unextracted samples
- QC check with Victor Nivo Multimode Microplate Reader
- Library preparation and sample indexing with Illumina kits
- Full automated library preparation on Hamilton NGS STAR
- Sequencing with NovaSeq X Plus or NovaSeq 6000, 30x genome coverage
- Turnover time approx. 2 to 3 weeks. Turnover times are not guaranteed, may change according to your project.
What we do with your samples
- Once your samples arrive at our laboratories, we start by performing a reception control step in which we make sure the samples meet our requirements.
- If the samples fail this quality control step, we will contact you to discuss possible options. Should you choose to proceed with samples not fulfilling the criteria it will be at your own risk, we will only make one attempt at library preparation.
- If the samples pass reception control, we will inform you and the samples will be queued for library preparation.
Library preparation
- Illumina’s new tagmentation-based DNA PCR-free library preparation is the method of choice for generating the highest quality DNA sequencing libraries. PCR-free libraries have better coverage of GC-rich regions compared to PCR-based methods and the reads are more evenly distributed over the genome.
- Library QC and sequencing: We evaluate the concentration of the library and inform you of the QC status of each sample. Once the libraries have passed this QC step, they are normalized, pooled, and queued for sequencing.
Sequencing & Analysis
- Raw sequencing data is first generated on either Illumina NovaSeq X Plus or NovaSeq 6000 instruments.
- Illumina DRAGEN Bio-IT Platform leverages for accurate, rapid secondary analysis
Output
- As a final output, we provide FASTQ files in tandem with BAM files as a standard set of output. If requested specifically by our clients, we can also provide VCF files.
- Final output files are shared via WeTransfer link.
๋ณธ ์๋น์ค๋ ๊ณ ๊ฐ๋์ ์ํ์ฑ ๋ถ์์ ์งํํ๋ ๋ง์ถคํ ์๋น์ค๋ก, ์ํ์ฑ ์์ ์ ํน์ฑ์ ๋ฐํ, ๊ตํ ๋ฐ ํ๋ถ์ด ์ ํ๋ฉ๋๋ค.
- ๋ฐํ ๋ฐ ํ๋ถ ๋ถ๊ฐ: ์ํ์ฑ ๋ถ์ ์๋น์ค๋ ์ฒซ QC(Quality Control) ์๋ฃ ํ ๋ผ์ด๋ธ๋ฌ๋ฆฌ ์ค๋น ์ , ๊ณ ๊ฐ๋์ ํ์ธ์ ๊ฑฐ์ณ ์งํ๋๋ฏ๋ก, ํด๋น ์์ ์ดํ์๋ ๋ฐํ ๋ฐ ํ๋ถ์ด ๋ถ๊ฐํฉ๋๋ค.
- ๊ตํ ์ ์ฑ : ์ํ์ฑ ์งํ ์ค ์๊ธฐ์น ์์ ํ์ง ๋ฌธ์ ๋ฐ์ ์, ๋ด๋ถ ๊ฒํ ๋ฅผ ๊ฑฐ์ณ ๋์ผํ ๋ถ์ ์กฐ๊ฑด์์ ์ฌ์งํ ๊ฐ๋ฅ ์ฌ๋ถ๋ฅผ ๊ฒํ ํฉ๋๋ค.
์๋น์ค ์ด์ฉ์ ๊ดํ ๋ฌธ์๋ ๊ณ ๊ฐ ์ง์ํ์ผ๋ก ์ฐ๋ฝ ์ฃผ์๊ธฐ ๋ฐ๋๋๋ค.
