SILK Longevity

Notifications

Application	Whole Genome Sequencing	Whole Exome Sequencing
Sample Type and Amount	(Blood) 650μL-1ml \| (gDNA) 50μL, >20 ng/μL in EB buffer (not EDTA/water) \| (Tissue) 30 - 50 mg \| (FFPE Tissue) 4-6 slices for 10µm, 8-10 slices for 5µm \| (Cell Pellet) 10^6 Euk. cells, 10^8 MO cells \| (Buccal Swab) Kit instruction \| (Saliva) 650μL-1mL If your samples are below the required thresholds, please get in touch.
Sample Requirement	(Storage and Transport) -80°C on dry ice (Concentration) >20 ng/μL using fluorometry (e.g. Qubit, Quant-it), not absorbance (Nanodrop, spectrophotometer) (Purity) A260/280: 1.8 - 2.0 and A260/230: 2.0 – 2.2
QC 1	Concentration and purity are measured using Victor Nivo Multiomode Microplate Reader and/or Agilent Bioanalyzer
Library Preparation	Illumina DNA PCR-Free Prep Kit on Hamilton NGS STAR	Illumina DNA Prep with Exome 2.5 Enrichment Kit on Hamilton NGS STAR
QC 2	qPCR and Bioanalyzer
Sequencing	Illumina NovaSeq X Plus (Depth ≥ 30X, 2x150 PE)	Illumina NovaSeq 6000 (8Gb/sample, Depth ≥ 100X)
Data Analysis	Illumina DRAGEN Germline on Bio-IT Platform	Illumina DRAGEN Enrichment on Bio-iT Platform
Final Output	(Data) FASTQ and MD5 (Report) Project Report

Euk : eukaryotic

MO : microorganism

Menu Layout